Khoom Description
Kev taw qhia thiab kev siv CAS:133876-92-3|Nattokinase
1. Kev ruaj ntseg ntawm Nattokinase
pH stability yog qhov ruaj khov nyob rau hauv lub ntuj pH nqi. Thaum pH tus nqi nce ntawm 7 mus rau 12, nws ruaj khov li ntawm 10 feeb ntawm chav tsev kub. Thaum tus nqi pH qis dua 5, nws tsis ruaj khov thiab poob haujlwm sai. Thaum qhov kub siab tshaj ntawm 60, nws sai sai ua tsis muaj zog vim protein denaturation. Tom qab tsib lub voj voog ntawm kev rov ua kom khov thiab thawing, ntau dua 95% ntawm nws cov haujlwm tseem raug khaws cia, qhia tias khov thiab thawing tsis muaj kev cuam tshuam loj rau kev ua ntawm nattokinase. Thaum nattokinase tov nrog boiled nplej extract, boiled broth, serum albumin thiab gastric mucus protein, lub stability ntawm nattokinase yog ho nce, thiab cov enzyme kev ua si tsis tag txawm nyob rau hauv acidic pH. Thermal stability: Thaum qhov kub qis dua 45 degree, qhov kev ua haujlwm yog qhov ruaj khov. Thaum qhov kub siab tshaj 60 degree, nws yog sai inactivated vim protein denaturation. Kev khov thiab thawing tsis muaj kev cuam tshuam loj rau kev ua haujlwm ntawm nattokinase. Tom qab rov ua kom khov thiab thawing 5 zaug, kev ua haujlwm ntawm nattokinase yog qhov ruaj khov. Cov enzyme tseem tuaj yeem tswj tau ntau dua 95% kev ua haujlwm. Hlau ion stability Cov kev ua ntawm nattokinase kuj cuam tshuam los ntawm qee cov hlau ions. Hg2+ yuav tsis ua haujlwm tag nrho. Cu2+, Zn2+, Al3+, Ca2+ thiab lwm yam ions muaj kev cuam tshuam sib txawv ntawm cov enzyme, thaum Mg2+ thiab Co{{16 }} muaj qhov sib txawv inhibitory cuam tshuam rau nws. ua kom cov nyhuv. Kev ruaj ntseg ntawm lwm yam tshuaj, E-ACA thiab EDTA, tsis muaj kev cuam tshuam loj rau kev ua haujlwm ntawm nattokinase, thaum PMSF tuaj yeem cuam tshuam tag nrho nws cov haujlwm. 1 mmol / L DFP thiab neguvon tuaj yeem cuam tshuam tag nrho cov haujlwm ntawm nattokinase.
2 molecular hnyav
Nattokinase yog ib leeg-chain polypeptidase. Vim muaj ntau txoj hauv kev los ntsuas qhov hnyav molecular, ntsuas qhov hnyav molecular kuj tseem txawv. Siv txoj kev SephadexG100 kem gel pom, cov txiaj ntsig tau txais yog 20, 000, thiab cov txiaj ntsig ntsuas los ntawm txoj kev ncig dichroic yog 27,300. Qhov hnyav molecular ntawm lub enzyme raug xam raws li cov amino acid ib ntus yog 27.728.
3.PI tus nqi (isoelectric point)
Siv Svunsson txoj kev kem electrophoresis thiab isoelectric focusing, nattokinase muaj ib tug symmetrical ib plasmin ncov, thiab nws PI tus nqi yog 8.6±0.3. Qhov taw tes isoelectric tuaj yeem muab tau. Thaum cais thiab purifying nattokinase, xav txog kev kho tus nqi pH ze ntawm tus nqi PI, uas muaj txiaj ntsig zoo rau kev ua kom huv ntawm enzyme.
4. Substrate tshwj xeeb ntawm nattokinase
Raws li ib qho serine protease, NK's amidolytic kev ua haujlwm tuaj yeem pom nrog ntau cov khoom siv hluavtaws. Lub substrate rhiab tshaj plaws rau nattokinase yog lub substrate ntawm plasma plasmin, uas yog S2251; Nws muaj qee yam kev ua haujlwm ntawm cov substrates H2DLArgPNA, S2238, S2266 thiab S2302, tab sis pom tseeb tsis ua haujlwm ntawm S2444 thiab S2484. Nattokinase muaj nws qhov tshwj xeeb amino acid ua haujlwm thiab paub qhov chaw rau hydrolyzing proteins.
Specification ntawm CAS:133876-92-3|Nattokinase
【Product specifications】 Nattokinase 20000FU/g
[Detection method] Phaj method
[Zoo] Off-dawb hmoov
[Packaging specifications] Txheem ntim: 25kg / cardboard nruas; bulk cargo: 100g / hnab
[Storage Conditions] kaw, txias, noo noo-pov thawj, tiv thaiv lub teeb
[Lub neej txee] 24 lub hlis
Pob ntawm CAS:133876-92-3|Nattokinase
25kg / cardboard nruas; bulk cargo: 100g / hnab
Cov khoom no siv tau rau 24 lub hlis txij li hnub tsim khoom thaum khaws cia hauv lub xeev sov so.
Kev nyab xeeb thiab kev thauj mus los
Cov khoom no yuav tsum tau muab ntim rau hauv chav tsev kub. Cov neeg siv khoom yuav tsum ua tib zoo nyeem cov khoom kho mob, cov ntaub ntawv kev nyab xeeb cov ntaub ntawv (MSDS) thiab cov ntawv sau ua ntej siv, paub txog cov khoom lag luam cov ntaub ntawv kev nyab xeeb, cov lus qhia ua haujlwm, kev cuam tshuam ib puag ncig, thiab hnav kev tiv thaiv tus kheej tsim nyog raws li qhov xav tau. khoom siv.
Cim npe nrov: cas:133876-92-3|nattokinase, Tuam Tshoj cas:133876-92-3|nattokinase manufacturers, hoobkas
