Kev piav qhia ntawm Folin-phenol reagent
| Purity | 99% |
| Pob | 50ML|100ML |
Product Description
1. Folin-phenol reagent A: (Yuav kom cov neeg siv khoom npaj lossis xaiv cov khoom siv sib xws)
Dissolve 1g Na2CO3 hauv 50mL 0.2moL/L NaOH, ces yaj 0.5g (CuSO4 5H2O) hauv 100mL 1% potassium sodium tartrate (los yog sodium tartrate) tov. Sib tov cov qub 50mL nrog rau tom kawg 1mL, npaj rau ntawm qhov chaw, thiab cov reagent no tsuas siv tau rau ib hnub, thiab nws yuav siv tsis tau tom qab tas sijhawm.
2. Folin-phenol reagent B:
Folin phenol feem ntau siv nyob rau hauv Lowry txoj kev rau tag nrho cov protein kom muaj nuj nqis
Qhov concentration ntawm no reagent yog 1moL / L, uas yog Folin-phenol reagent daim ntawv thov kua.
Lub hauv paus ntsiab lus ntawm Folin-phenol txoj kev rau kev txiav txim:
Nyob rau hauv alkaline tej yam kev mob, cov proteins reacts nrog tooj liab los tsim cov protein-tooj liab complexes. Cov complexes raug txo los ntawm Folln reagent (phosphomolybdic acid thiab phosphotungstic acid), thiab cov sib tov sib sib zog nqus xiav (ib tug sib tov ntawm phosphomolybdophilia thiab phosphotungsitophilia), xim tob yog proportional rau cov protein cov ntsiab lus. Txoj kev no yooj yim rau kev khiav lag luam thiab 100 lub sij hawm ntau rhiab dua li txoj kev bipyridyl. Qhov ntau npaum li cas yog 5 ~ 100 ug ntawm cov protein.
Cim npe nrov: Tuam Tshoj folin-phenol reagent manufacturers, hoobkas
