Taw qhia ntawm CAS 39450-01-6|Proteinase K
Proteinase K= Protein (ntev saw ntawm amino acid) + ase (suffix rau enzyme) + K (keratin)
Proteinase K yog ib qho nquag protease ntawm cov chav kawm subtilisin, siv rau kev degradation ntawm cov proteins hauv cov qauv lom neeg. , muaj qhov zoo ntawm kev ua haujlwm siab, siab tawm los thiab dav kub / pH kev ua haujlwm ntau.
Lub luag haujlwm ntawm proteinase K digestion hauv DNA extraction
Cov proteinase K digests feem ntau yog hydrophobic amino acids (ob qho tib si aromatic thiab aliphatic). Cov cofactor, calcium ion muab kev ruaj ntseg rau cov enzyme, txawm li cas los xij, nws tsis tuaj yeem ua rau cov tshuaj tiv thaiv.
Cov proteinase K siab tshaj plaws tau tshaj tawm ntawm qhov kub ntawm 60ºC txij li 37ºC txog 70ºC. Qhov ntsuas kub thiab qhov ua haujlwm enzymatic daim duab qhia hauv qab no.
Daim ntawv thov CAS 39450-01-6|Proteinase K
Siv rau hauv cov khoom siv tshuaj ntsuam genetic, genomic DNA extraction kits, thiab RNA extraction kits kom tshem tawm cov nucleases thiab lwm yam kab mob protein;
Kev rho tawm cov khoom tsis muaj protein ntau xws li cov tshuaj nucleic acid thiab polysaccharide hauv kev lag luam biopharmaceutical, xws li kev npaj cov tshuaj tiv thaiv DNA thiab heparin; Siv nyob rau hauv kev lag luam niaj hnub xws li kev kho mob ntawm daim tawv nqaij, tshem tawm cov laus cuticle hlwb, tanning ntawm daim tawv nqaij, npaj hydrolyzed protein seasoning, tshuaj pleev ib ce thiab pub noj.
[Enzyme kev ua haujlwm] Ntau dua lossis sib npaug rau 30U / mg cov hmoov qhuav qhuav
[Qhov zoo tshaj pH ntau] 7.5-11.5
[Microbiological txwv] Tsawg dua lossis sib npaug li 5 cfu / g
[Qhov kub zoo tshaj plaws] Qhov ntsuas kub tau pom zoo yog 37-70 degree, nrog rau qhov siab tshaj plaws ntawm 70 degree
[Inactivation method] Ntxiv 5mM PMSF rau cov tshuaj tiv thaiv kom inactivate nws.
[Inhibitors] Diisopropylfluorophosphate (DIFP), phenylmethylsulfonyl fluoride (PMSF)
Specification ntawm CAS 39450-01-6|Proteinase K
|
Cov khoom |
Specification |
|
Molecular Luj |
29.7kd ua |
|
Qhov tshwm sim |
Lyophilized hmoov |
|
Cia tej yam kev mob |
Khaws hauv qab 4 degree |
|
Purity |
Tus nqi, 99% |
|
Kev ua haujlwm enzyme |
Ntau dua lossis sib npaug li 30U / mg qhuav hnyav |
|
DNA & RNA Contamination Assay |
Tsis pom muaj leej twg |
|
DNase Contamination Assay |
Tsis pom muaj leej twg |
|
RNase Contamination Assay |
Tsis pom muaj leej twg |
Khoom Qeb Proteinase K
|
Khoom npe
|
Qhov tshwm sim | Kev ua haujlwm enzyme | Ntim specifications |
| Proteinase K khov-qhuav hmoov | Dawb los yog dawb hmoov hmoov | Ntau dua lossis sib npaug li 30U / mg | 20mg / lub raj mis, 1g / lub raj mis, 10g / lub raj mis, thiab lwm yam. |
| Proteinase K sterile freeze-dried hmoov | Dawb los yog dawb hmoov hmoov | Ntau dua lossis sib npaug li 30U / mg | 100mg / lub raj mis, 100g / lub raj mis, thiab lwm yam. |
| Proteinase K kua (20mg / ml) | Colorless pob tshab tov | Ntau dua lossis sib npaug li 900U / ml | 1ml / lub raj mis, 10ml / lub raj mis, 1L / lub raj mis, thiab lwm yam. |
| Proteinase K kua (40mg / ml) | Colorless pob tshab tov | Ntau dua lossis sib npaug rau 1800U / ml | 1ml / lub raj mis, 10ml / lub raj mis, 1L / lub raj mis, thiab lwm yam. |
| Muaj qib Proteinase K | Lub teeb daj khov-dryed hmoov | Ntau dua lossis sib npaug li 150U / g | 10g / lub raj mis, 20g / lub raj mis, 50g / lub raj mis, thiab lwm yam. |
20mg / lub raj mis, 100mg / lub raj mis, 1g / lub raj mis, 10g / lub raj mis, tau ntim raws li cov neeg siv khoom xav tau
Khaws lub tub yees hauv qab 4 degree
Cov tshuaj Proteinase K
Cim npe nrov: cas 39450-01-6 proteinase k, Tuam Tshoj cas 39450-01-6 proteinase k manufacturers, hoobkas
